unravelling_fig4.jpg

Gamete surface immuno-fluorescence assay (SIFA) using wild-type and Pfs48/45 knockout (KO) NF54 gametes, with Pfs48/45 mAb, Pfs230 mAb, and IgG from a malaria-exposed serum donor. Donor IgG is from donor A in Table 2, and was performed using total IgG, and total IgG depleted of α-Pfs48/45-10c and α-Pfs230 (230CMB) IgG. Δ Pfs48/45 = Pfs48/45 KO3. BF Bright-field, FITC fluorescein isothiocyanate. Anti-Pfs48/45 is mAb 45.1, and anti-Pfs230 is mAb 2A2, as described in the methods. Scale bar is 20 µm